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BMC Microbiol. 2006; 6: 16.
Published online 2006 February 24. doi: 10.1186/1471-2180-6-16.
Expression of hemagglutinin protein from the avian influenza virus H5N1 in a baculovirus/insect cell system significantly enhanced by suspension culture
Nitar Nwe,1 Qigai He,1 Sudarat Damrongwatanapokin,2 Qingyun Du,1 Ivanus Manopo,1 Yukol Limlamthong,2 Beau James Fenner,1 Lynn Spencer,1 and Jimmy Kwang1
1Animal Health Biotechnology Group, Temasek Life Science Laboratory, 1 Research Link, National University of Singapore, Singapore 117604, Singapore
2Department of Livestock Development, National Institute of Animal Health, Chatuchak, Bangkok, Thailand
Corresponding author.
Nitar Nwe: **********; Qigai He: **********; Sudarat Damrongwatanapokin: **********; Qingyun Du: **********; Ivanus Manopo: **********; Yukol Limlamthong: **********; Beau James Fenner: **********; Lynn Spencer: **********; Jimmy Kwang: **********
Received August 19, 2005; Accepted February 24, 2006.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Background
Prevention of a possible avian influenza pandemic necessitates the development of rapid diagnostic tests and the eventual production of a vaccine.
Results
For vaccine production, hemagglutinin (HA1) from avian influenza H5N1 was expressed from a recombinant baculovirus. Recombinant HA1 was expressed in monolayer or suspension culture insect cells by infection with the recombinant baculovirus. The yield of rHA1 from the suspension culture was 68 mg/l, compared to 6 mg/l from the monolayer culture. Immunization of guinea pigs with 50 μg of rHA1 yielded hemagglutinin inhibition and virus neutralization titers of 1:160 after two times vaccination with rHA1 protein.
Conclusion
Thus, the production of rHA1 using an insect suspension cell system provides a promising basis for economical production of a H5 antigen.
Published online 2006 February 24. doi: 10.1186/1471-2180-6-16.
Expression of hemagglutinin protein from the avian influenza virus H5N1 in a baculovirus/insect cell system significantly enhanced by suspension culture
Nitar Nwe,1 Qigai He,1 Sudarat Damrongwatanapokin,2 Qingyun Du,1 Ivanus Manopo,1 Yukol Limlamthong,2 Beau James Fenner,1 Lynn Spencer,1 and Jimmy Kwang1
1Animal Health Biotechnology Group, Temasek Life Science Laboratory, 1 Research Link, National University of Singapore, Singapore 117604, Singapore
2Department of Livestock Development, National Institute of Animal Health, Chatuchak, Bangkok, Thailand
Corresponding author.
Nitar Nwe: **********; Qigai He: **********; Sudarat Damrongwatanapokin: **********; Qingyun Du: **********; Ivanus Manopo: **********; Yukol Limlamthong: **********; Beau James Fenner: **********; Lynn Spencer: **********; Jimmy Kwang: **********
Received August 19, 2005; Accepted February 24, 2006.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Background
Prevention of a possible avian influenza pandemic necessitates the development of rapid diagnostic tests and the eventual production of a vaccine.
Results
For vaccine production, hemagglutinin (HA1) from avian influenza H5N1 was expressed from a recombinant baculovirus. Recombinant HA1 was expressed in monolayer or suspension culture insect cells by infection with the recombinant baculovirus. The yield of rHA1 from the suspension culture was 68 mg/l, compared to 6 mg/l from the monolayer culture. Immunization of guinea pigs with 50 μg of rHA1 yielded hemagglutinin inhibition and virus neutralization titers of 1:160 after two times vaccination with rHA1 protein.
Conclusion
Thus, the production of rHA1 using an insect suspension cell system provides a promising basis for economical production of a H5 antigen.